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Index > Protein center > Ppargc1a(Gene name) > Mouse
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  • Ppargc1a (Gene name),
  • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (Protein name ),  PRGC1_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Ppargc1a(Pgc1;Pgc1a;Ppargc1);
    Protein name:
    Peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC-1-alpha;PPAR-gamma coactivator 1-alpha;PPARGC-1-alpha);
    Alternative:

    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Homooligomer (By similarity). Binds MYBBP1A, which inhibits transcriptional activation by this protein. Interacts with LRPPRC (By similarity). Interacts with PRDM16. Interacts with LPIN1.
    Function:
    Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis.
    Subcellular Location:
    Nucleus
    Protein Attributes:
    Sequence length:
    797
    Sequence:
    50:
    MAWDMCSQDS | VWSDIECAAL | VGEDQPLCPD | LPELDLSELD | VNDLDTDSFL | 
    100:
    GGLKWCSDQS | EIISNQYNNE | PANIFEKIDE | ENEANLLAVL | TETLDSLPVD | 
    150:
    EDGLPSFDAL | TDGAVTTDNE | ASPSSMPDGT | PPPQEAEEPS | LLKKLLLAPA | 
    200:
    NTQLSYNECS | GLSTQNHAAN | HTHRIRTNPA | IVKTENSWSN | KAKSICQQQK | 
    250:
    PQRRPCSELL | KYLTTNDDPP | HTKPTENRNS | SRDKCASKKK | SHTQPQSQHA | 
    300:
    QAKPTTLSLP | LTPESPNDPK | GSPFENKTIE | RTLSVELSGT | AGLTPPTTPP | 
    350:
    HKANQDNPFK | ASPKLKPSCK | TVVPPPTKRA | RYSECSGTQG | SHSTKKGPEQ | 
    400:
    SELYAQLSKS | SGLSRGHEER | KTKRPSLRLF | GDHDYCQSLN | SKTDILINIS | 
    450:
    QELQDSRQLD | FKDASCDWQG | HICSSTDSGQ | CYLRETLEAS | KQVSPCSTRK | 
    500:
    QLQDQEIRAE | LNKHFGHPCQ | AVFDDKSDKT | SELRDGDFSN | EQFSKLPVFI | 
    550:
    NSGLAMDGLF | DDSEDESDKL | SYPWDGTQPY | SLFDVSPSCS | SFNSPCRDSV | 
    600:
    SPPKSLFSQR | PQRMRSRSRS | FSRHRSCSRS | PYSRSRSRSP | GSRSSSRSCY | 
    650:
    YYESSHYRHR | THRNSPLYVR | SRSRSPYSRR | PRYDSYEAYE | HERLKRDEYR | 
    700:
    KEHEKRESER | AKQRERQKQK | AIEERRVIYV | GKIRPDTTRT | ELRDRFEVFG | 
    750:
    EIEECTVNLR | DDGDSYGFIT | YRYTCDAFAA | LENGYTLRRS | NETDFELYFC | 
    797:
    GRKQFFKSNY | ADLDTNSDDF | DPASTKSKYD | SLDFDSLLKE | AQRSLRR
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    String:
    UniGene:
    Pfam:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse PGC-1-alpha
    Cat.:
    E1160h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse PGC-1-alpha
    Cat.:
    E1160r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse PGC-1-alpha
    Cat.:
    E1160m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse PGC-1-alpha
    Cat.:
    E1160p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse PGC-1-alpha
    Cat.:
    E1160b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse PGC-1-alpha
    Cat.:
    U1160r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse PGC-1-alpha
    Cat.:
    U1160h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse PGC-1-alpha
    Cat.:
    U1160m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse PGC-1-alpha
    Cat.:
    U1160p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse PGC-1-alpha
    Cat.:
    U1160b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Mouse PGC-1-alpha
    Cat.:
    P1160Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse PGC-1-alpha
    Polyclonal Antibody for Mouse PGC-1-alpha
    Cat.:
    P1160Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse PGC-1-alpha
    Cat.:
    P1160Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse PGC-1-alpha
    Monoclonal Antibody for Mouse PGC-1-alpha
    Monoclonal Antibody for Mouse PGC-1-alpha
    Monoclonal Antibody for Mouse PGC-1-alpha
    Monoclonal Antibody for Mouse PGC-1-alpha
    Monoclonal Antibody for Mouse PGC-1-alpha
    Protein for Mouse PGC-1-alpha
    Protein for Mouse PGC-1-alpha
    Protein for Mouse PGC-1-alpha
    Protein for Mouse PGC-1-alpha
    Protein for Mouse PGC-1-alpha

    R&D Technical Data
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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis."
      Puigserver P. , Wu Z. , Park C.W. , Graves R. , Wright M. , Spiegelman B.M.
      Cell92:829-839(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;INDUCTION;TISSUE SPECIFICITY
      strain: C57BL/6J.
      tissue: Brown adipose tissue.
    2. 2.
      "A PGC-1alpha isoform induced by resistance training regulates skeletal muscle hypertrophy."
      Ruas J.L. , White J.P. , Rao R.R. , Kleiner S. , Brannan K.T. , Harrison B.C. , Greene N.P. , Wu J. , Estall J.L. , Irving B.A. , Lanza I.R. , Rasbach K.A. , Okutsu M. , Nair K.S. , Yan Z. , Leinwand L.A. , Spiegelman B.M.
      Cell151:1319-1331(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4);FUNCTION;SUBCELLULAR LOCATION;ALTERNATIVE PROMOTER USAGE;TISSUE SPECIFICITY;INDUCTION
      strain: C57BL/6.
      tissue: Skeletal muscle.
    3. 3.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      strain: C57BL/6J.
      tissue: Spinal cord.
      tissue: Spleen.
    4. 4.
      Mural R.J. , Adams M.D. , Myers E.W. , Smith H.O. , Venter J.C.
      Submitted (2005-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    5. 5.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      strain: CD-1.
      tissue: Neural stem cell.
    6. 6.
      "Role of leptin in peroxisome proliferator-activated receptor gamma coactivator-1 expression."
      Kakuma T. , Wang Z.-W. , Pan W. , Unger R.H. , Zhou Y.-T.
      Endocrinology141:4576-4582(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
    7. 7.
      "Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK."
      Fan M. , Rhee J. , St Pierre J. , Handschin C. , Puigserver P. , Lin J. , Jaeger S. , Erdjument-Bromage H. , Tempst P. , Spiegelman B.M.
      Genes Dev.18:278-289(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MYBBP1A
    8. 8.
      "Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1."
      Rodgers J.T. , Lerin C. , Haas W. , Gygi S.P. , Spiegelman B.M. , Puigserver P.
      Nature434:113-118(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ACETYLATION AT LYS-77; LYS-144; LYS-183; LYS-253; LYS-270; LYS-277; LYS-320; LYS-346; LYS-412; LYS-441; LYS-450; LYS-757 AND LYS-778;DEACETYLATION BY SIRT1
    9. 9.
      "Lipin 1 is an inducible amplifier of the hepatic PGC-1alpha/PPARalpha regulatory pathway."
      Finck B.N. , Gropler M.C. , Chen Z. , Leone T.C. , Croce M.A. , Harris T.E. , Lawrence J.C. Jr. , Kelly D.P.
      Cell Metab.4:199-210(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LPIN1
    10. 10.
      "Transcriptional control of brown fat determination by PRDM16."
      Seale P. , Kajimura S. , Yang W. , Chin S. , Rohas L.M. , Uldry M. , Tavernier G. , Langin D. , Spiegelman B.M.
      Cell Metab.6:38-54(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PRDM16
    11. 11.
      "AMP-activated protein kinase (AMPK) action in skeletal muscle via direct phosphorylation of PGC-1alpha."
      Jager S. , Handschin C. , St-Pierre J. , Spiegelman B.M.
      Proc. Natl. Acad. Sci. U.S.A.104:12017-12022(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-177 AND SER-538;MUTAGENESIS OF THR-177 AND SER-538
    12. 12.
      "Transcriptional coactivator PGC-1alpha integrates the mammalian clock and energy metabolism."
      Liu C. , Li S. , Liu T. , Borjigin J. , Lin J.D.
      Nature447:477-481(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CIRCADIAN RHYTHMS;INTERACTION WITH RORA AND RORC;TISSUE SPECIFICITY;INDUCTION;DISRUPTION PHENOTYPE;MUTAGENESIS OF 142-LEU--LEU-146
    13. 13.
      "Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex."
      Kajimura S. , Seale P. , Tomaru T. , Erdjument-Bromage H. , Cooper M.P. , Ruas J.L. , Chin S. , Tempst P. , Lazar M.A. , Spiegelman B.M.
      Genes Dev.22:1397-1409(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PRDM16
    14. 14.
      "Cdc2-like kinase 2 is an insulin-regulated suppressor of hepatic gluconeogenesis."
      Rodgers J.T. , Haas W. , Gygi S.P. , Puigserver P.
      Cell Metab.11:23-34(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION BY CLK2
    15. 15.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH PML
    16. 16.
      "A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis."
      Bostrom P. , Wu J. , Jedrychowski M.P. , Korde A. , Ye L. , Lo J.C. , Rasbach K.A. , Bostrom E.A. , Choi J.H. , Long J.Z. , Kajimura S. , Zingaretti M.C. , Vind B.F. , Tu H. , Cinti S. , Hojlund K. , Gygi S.P. , Spiegelman B.M.
      Nature481:463-468(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
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